RESUMO
BACKGROUND AND PURPOSE: Fusariosis is a fungal infection often involving the skin. Various species can cause local, focally invasive, or disseminated infections. The routes of entry for Fusarium species include the respiratory tract, gastrointestinal tract, toe nails, trauma to the skin, and indwelling central venous catheter. CASE REPORT: Herein, we present the case of a 35-year-old woman presenting with interdigital intertrigo. The patient had no predisposing factors and she did not take any antifungal agents. Fusiform macroconidia were observed on the slide culture of the fungus. The etiological agent of the infection was identified as Fusarium oxysporum through sequencing of the translation elongation factor-1 alpha (TEF-1α) gene using the primers EF1 and EF2. CONCLUSION: Fusariosis commonly presents as a severe fungal infection in immunocompromised patients. However, this infection may also occur in immunocompetent patients. Although treatment with amphotericin B is a routine antifungal therapy for fusariosis, many azoles such as cloterimazole can be used topically with fewer side-effects.
RESUMO
A rapid, simple and sensitive assay was developed for determination of captopril in human serum. We employed silica gel cartridge for efficient extraction of captopril adduct from human serum. Captopril was trapped with p-bromophenacyl bromide (pBPB) to give captopril-pBPB adduct. A 4-ml benzene extract of 1 ml acidified serum was passed through 1 ml silica gel cartridge. Potential interfering compounds were removed with 4-ml benzene wash. The captopril-pBPB adduct was eluted with 0.5 ml acetonitrile. Of this acetonitrile solution (100 microliters) was injected on an ODS reverse phase HPLC column (chromatography conditions; mobile phase; acetonitrile-water-acetic acid (225:270:5, v/v/v), flow rate; 1 ml min-1, detection; UV at 263 nm). It is found that this method is accurate and does not require time consuming evaporation-concentration steps. Recovery exceeds 94% and analytical responses are linear over captopril concentration range from 50 up to 1000 ng ml-1. The coefficients of variation from 108 ng ml-1 to 605 ng ml-1 varied between 3.7-7.7% and the relative error did not exceed 3.7%. Therefore, this method can be used for routine clinical monitoring and in pharmacokinetic studies of captopril.
